Gladius WGS vs IWGSC RefSeq v1.0 Genome Assembly

WGS reads of Gladius (http://dx.doi.org/10.1111/j.1467-7652.2012.00717.x) were aligned against the IWGSC Triticum aestivum Chinese Spring RefSeq v1.0 genome assembly using Minimap2. These BAM files (.bam) and index files (.bam.bai) are provided together with a summary of read alignment coverage bigWig files (.bam.bw). SNP variants were called from these BAM files to generate VCF files (.bam.vcf.gz) and index files (.bam.vcf.gz.tbi) and are provided together with a summary of SNP density (SNPs per 10 kbp) bigWig files (.bam.vcf.w10000_s10000.bw). VCF files contain the following filter values and corresponding meaning: PASS = high quality (Q>=30) homozygous; Het = high quality (Q>=30) heterozygous; LowQualHom = low quality (Q

本数据集包含来自Gladius的全基因组测序（Whole Genome Sequencing, WGS）读段，相关文献DOI链接为http://dx.doi.org/10.1111/j.1467-7652.2012.00717.x。该测序读段已通过Minimap2软件比对至国际小麦基因组测序联盟（International Wheat Genome Sequencing Consortium, IWGSC）发布的普通小麦中国春参考基因组组装序列IWGSC Triticum aestivum Chinese Spring RefSeq v1.0。本数据集附带比对生成的BAM格式文件（.bam）及其索引文件（.bam.bai），同时提供了测序读段比对覆盖度汇总的bigWig格式文件（.bam.bw）。研究人员从上述BAM文件中调用单核苷酸多态性（Single Nucleotide Polymorphism, SNP）变异，生成了变异呼叫格式（Variant Call Format, VCF）压缩文件（.bam.vcf.gz）及其索引文件（.bam.vcf.gz.tbi），并附带了SNP密度（每10千碱基对的SNP数目）汇总的bigWig格式文件（.bam.vcf.w10000_s10000.bw）。上述VCF文件包含以下过滤标签及其对应含义：PASS代表质量值Q≥30的纯合变异；Het代表质量值Q≥30的杂合变异；LowQualHom代表低质量（Q