Human Sperm Head Morphology dataset (HuSHeM)

At the Isfahan Fertility and Infertility Center, semen samples were collected from fifteen patients. The sperm samples were fixed and stained using the Diff-Quick method. Using an Olympus CX21 microscope with a ×100 objective lens and a ×10 eyepiece and a Sony color camera (Model No SSC-DC58AP), 725 images were taken. The resolution of each image was 576×720 pixels. From these images, the sperm heads were cropped and classified into five classes by three specialists. The classes are Normal, Pyriform, Tapered, Amorphous, and Others. After the classification, only the samples which there was a collective consensus about their class were kept in the dataset. Four classes of Normal, Pyriform, Tapered, and Amorphous are included in this dataset. The resulting dataset of sperm heads denoted as Human Sperm Head Morphology dataset (HuSHeM) consists of four folders, each corresponding to a specific set of sperm shapes. The folder names reflect the shape of the contained images. There are 54 Normal, 53 Tapered, 57 Pyriform, and 52 Amorphous sperm heads. The images of sperm heads are in the RGB format with the size of 131×131 pixels.

本数据集采集自伊斯法罕生殖与不孕诊疗中心，从15名患者处收集精液样本。精子样本经固定后采用迪夫快速染色法（Diff-Quick）完成染色。使用配备100倍物镜与10倍目镜的奥林巴斯CX21显微镜，搭配型号为SSC-DC58AP的索尼彩色相机，共采集725幅图像，单幅图像分辨率为576×720像素。研究人员从上述图像中裁剪得到精子头部图像，并由3名专业医师将其划分为5个类别，分别为正常（Normal）、梨形（Pyriform）、锥形（Tapered）、不规则形（Amorphous）及其他类别。分类完成后，仅保留3名医师就其类别达成一致共识的样本，纳入本数据集。本数据集最终仅包含正常、梨形、锥形及不规则形4个类别。本次构建的人类精子头部数据集被命名为人类精子头部形态学数据集（Human Sperm Head Morphology dataset, HuSHeM），共包含4个文件夹，每个文件夹对应一类特定的精子头部形态，文件夹名称直接反映其存储图像的精子头部形态类别。各分类下的样本数量分别为：正常头部54例、锥形头部53例、梨形头部57例、不规则形头部52例。所有精子头部图像均为RGB格式，分辨率为131×131像素。