Discovery of YAP target genes with TNFa or IL-1b induction

Proper inflammatory responses are vital to restore tissue homeostasis against offending factors. However, it is largely under explored whether inflammatory responses are modulated by the Hippo effectors YAP/TAZ. To identify the transcription targets related to inflammatory reponses for YAP, we utilized human bronchioalveolar carcinoma cells H358 expressing YAP(5SA) or GFP (as a control) and treated with vehicle, TNFa or IL-1b, respectively. Our work disclosed the complex features of YAP/TAZ’s role in inflammatory responses. H358 GFP and H358 YAP(5SA) are generated. Each cell line was treated with 10 g/ml TNF-a, 10 g/ml IL-1b or vehicle for 1 hour. This makes 6 samples. The expression data from H358 GFP treated with vehicle serve as control.