Impact of common sample pre-treatments on key soil microbial properties

Data and R scripts to the associated open access publication in Soil Biology and Biochemistry. Based on five different paired sites (each with cropland and forest), the effects of six common pre-treatments were evaluated on 18O-CUE and associated parameters, i.e. respiration rate, soil microbial biomass C, total DNA extracted, and estimated abundances of fungi, bacteria and archaea. The six common pre-treatments were: (i) immediate analysis of field-fresh soil samples, and analysis following 14 d pre-incubation of soil samples that were (ii) field-fresh, (iii) air-dried, (iv) oven-dried (40 °C), (v) frozen at -20°C and (vi) frozen in situ (dry ice and subsequently liquid N2). Please read info.txt for information on the content of individual files.本数据集配套发表于《Soil Biology and Biochemistry》（土壤生物学与生物化学）的开放获取论文的相关数据与R脚本。 本数据集基于5组不同的配对样地（每组均包含农田与森林两类样地），评估了6种常用预处理方式对18O碳利用效率（18O-CUE）及相关参数的影响，相关参数即呼吸速率、土壤微生物生物量碳、提取的总DNA，以及真菌、细菌与古菌的估算丰度。6种常用预处理方式分别为：(i) 对野外新鲜土壤样品直接进行分析；以及对经14天预培养后的土壤样品进行分析，此类预培养样品又分为：(ii) 野外新鲜状态、(iii) 风干、(iv) 40℃烘箱烘干、(v) -20℃冷冻保存、(vi) 原位冷冻（先用干冰，随后使用液氮）。 请查阅info.txt文件以了解各单个文件的内容说明。