Data_Sheet_1_Phylogenetics and Mitogenome Organisation in Black Corals (Anthozoa: Hexacorallia: Antipatharia): An Order-Wide Survey Inferred From Complete Mitochondrial Genomes.pdf

Black corals (Anthozoa: Antipatharia) are an ecologically and culturally important group of deep-sea cnidarians. However, as the majority of species inhabit depths >50 m, they are relatively understudied. The inaccessibility of well-preserved tissue for species of interest has limited the scope of molecular analysis, and as a result only a small number of antipatharian mitochondrial genomes have been published. Using next generation sequencing, 18 complete and five partial antipatharian mitochondrial genomes were assembled, increasing the number of complete mitochondrial genomes to 22. This includes species from six antipatharian families, four of which were previously unrepresented, enabling the first family-level, full mitochondrial gene analysis over the whole order. The circular mitogenomes ranged in size from 17,681 to 21,669 bp with the large range in size due to the addition of an intron in COX1 in some species and size variation of intergenic regions. All mitogenomes contained the genes standard to all hexacoral mitogenomes (13 protein coding genes, two rRNAs and two tRNAs). The only difference in gene content is the presence of the COX1 intron in five families. The most variable mitochondrial gene is ND4 which may have implications for future barcoding studies. Phylogenetic analysis confirms that Leiopathidae is sister to all other families. Families Antipathidae, Cladopathidae and Schizopathidae are polyphyletic, supporting previous studies that call for a taxonomic revision.

黑色珊瑚（珊瑚纲：海葵目）是一群在生态和文化上具有重要意义的深海刺胞动物。然而，由于大部分物种栖息在超过50米的深度，它们相对较少被研究。对于目标物种而言，难以获取保存完好的组织限制了分子分析的范围，因此，公开发表的抗珊瑚线粒体基因组数量寥寥无几。通过下一代测序技术，我们组装了18个完整的和5个部分的抗珊瑚线粒体基因组，使完整的线粒体基因组数量增至22个。这包括来自六个抗珊瑚科的代表物种，其中四个科之前未曾有代表物种被记录，从而实现了整个目层次上的首次家族级全线粒体基因分析。这些环状线粒体基因组的大小介于17,681至21,669碱基对之间，这种大小的差异主要是由于某些物种中COX1基因内含子的添加以及基因间区域大小的变化。所有线粒体基因组均包含所有六放珊瑚线粒体基因组中标准的基因（13个编码蛋白基因，两个核糖体RNA和两个转运RNA）。唯一的不同之处在于五个科中存在COX1基因内含子。最易变异的线粒体基因是ND4，这可能会对未来条形码研究产生影响。系统发育分析证实，莱伊氏珊瑚科是所有其他科的同源科。抗珊瑚科、枝状珊瑚科和裂状珊瑚科是多系的，支持先前的研究，呼吁进行分类修订。