Warming and reduced rainfall alter fungal necromass decomposition rates and associated microbial community composition and functioning at a temperate-boreal forest ecotone

Changes in temperature and rainfall associated with altered climatic conditions are likely to significantly alter rates of soil organic matter decomposition. To determine how the combined effects of warming and drought impact the decomposition of fungal necromass, a large and fast-cycling portion of the global soil organic carbon (C) pool, we incubated Hyaloscypha bicolor necromass under both ambient and altered conditions (air and soil warming +3.3°C and ~40% reduced rainfall) at the B4Warmed experiment in Minnesota, USA. We conducted two multi-week incubations, one assessing mass loss and microbial community composition on decaying necromass after 1, 2, 7, and 14 weeks and the second characterizing the substrate utilization capacities of necromass-associated microbial communities after weeks 1 and 7. Warming and reduced rainfall accelerated the initial rate of necromass decay by ~20%, yet slowed overall mass loss by ~6% at the end of the 14 week incubation. These different rates of de..., , # Warming and reduced rainfall alter fungal necromass decomposition rates and associated microbial community composition and functioning at a temperate-boreal forest ecotone

Dataset DOI: [10.5061/dryad.rn8pk0pp6](10.5061/dryad.rn8pk0pp6)

## Description of the data and file structure

This data was collected to determine the impact that experimental warming and reduced rainfall has on decomposing fungal necromass in a temperate-boreal ecotone. We incubated fungal necromass and determined mass loss across time (1, 2, 7, and 14 weeks), sequenced the associated bacterial and fungal communities, and assessed microbial substrate utilization using biolog eco plates.

### Files and variables

#### File: B4W_Necro_MassLoss.csv

**Description:** Mass loss measurements for the 14-week incubation. Missing values are labeled as NA.

##### Variables

* Plot : Plot ID based on 3 different blocks.
* Plot_ID: The ID of the bag tags.
* set: Respective set associated with each group of bags.
* Treatment...,

气候变化引发的气温与降水改变，极有可能显著调控土壤有机质的分解速率。为明确增温与干旱的联合效应如何影响真菌残体（fungal necromass）的分解——真菌残体是全球土壤有机碳（soil organic carbon (C)）库中体量庞大且周转快速的组分——我们于美国明尼苏达州的B4Warmed野外试验平台，在对照与改变后的气候条件（空气与土壤增温3.3℃，降水减少约40%）下，开展了双色丝膜盘菌（Hyaloscypha bicolor）残体的培养实验。

本研究共开展两项为期数周的培养实验：其一用于评估1、2、7及14周时腐解残体的质量损失与微生物群落组成；其二则用于表征第1周和第7周时，与残体定殖的微生物群落的底物利用能力。

实验结果显示：增温与降水减少可使真菌残体的初始分解速率提升约20%，但在14周培养结束时，却使整体质量损失降低约6%。这类迥异的分解速率……
# 增温与降水减少改变温带-寒带森林交错带（temperate-boreal forest ecotone）中真菌残体的分解速率及其伴随微生物群落的组成与功能

数据集DOI：10.5061/dryad.rn8pk0pp6

## 数据与文件结构说明
本数据集旨在探究实验性增温与降水减少对温带-寒带森林交错带内腐解真菌残体的影响。我们对真菌残体进行培养，测定了不同时间节点（1、2、7及14周）的质量损失，对伴随的细菌与真菌群落进行测序，并利用Biolog Eco板（Biolog Eco plate）评估了微生物的底物利用能力。

### 文件与变量
#### 文件：B4W_Necro_MassLoss.csv
**描述**：14周培养实验的质量损失测定数据，缺失值以NA标注。
##### 变量：
* Plot：基于3个不同样地分区的样地编号
* Plot_ID：培养袋标签的编号
* set：对应每组培养袋的实验分组
* Treatment……