Gene expression of osteosarcama cell line with vasculogenic mimicry phenotype

Vasculogenic mimicry has been generally accepted as a new form of tumor vascularization and regarded as an unfavorable prognostic factor in multiple aggressive malignancies. We previously reported the presence of vasculogenic mimicry in osteosarcoma patients. The mechanistic basis for osteosarcoma VM remains unclear. We used microarrays to detail the global programme of gene expression between 143B cells and HOS cells exposed to Matrigel which showed greatly different vasculogenic mimicry formation potential and identified distinct classes of vasculogenic mimicry-realted genes during this process. Osteosarcoma cell lines 143B and HOS cultured in Matrigel after 24 hours were obtained for RNA extraction and hybridization on Affymetrix microarrays. There are duplicate samples for each cell line.