DATA SET FOR QUANTIFICATION OF TOTAL PHENOLIC COMPOUNDS, FLAVONOIDS, ANTIOXIDANT ACTIVITY, IN VITRO ENZYMATIC INHIBITION AND HEMOLYTIC ACTIVITY OF ANDIROBA (CARAPA GUIANENSIS AUBL) INDUSTRIAL WASTE

This study demonstrated the bioactive potential of the ethanolic extract of the industrial residue of andiroba (Carapa guianensis), focusing on its pharmaceutical applicability in enzyme inhibition. Analyses were performed to quantify total phenols and flavonoids, in addition to in vitro assays to evaluate the antioxidant activity by the DPPH and FRAP methods and the inhibition of the enzymes α-amylase and lipase. The amount of phenols and flavonoids in the sample did not show significant results. However, the extract showed antioxidant activity by the DPPH method, with inhibition of 45.16 ± 7.43% at a concentration of 10 mg/mL and IC50 of 11.57 ± 1.05 mg/mL. The FRAP method showed antioxidant capacity, with inhibition of 238.90 ± 8.78% and IC₅₀ of 0.51 ± 0.07 mg/mL. Furthermore, the extract showed α-amylase inhibitory activity, reaching 65.38% inhibition at 25 mg/mL, with IC₅₀ of 17.52 ± 4.57 mg/mL. The extract also demonstrated inhibition of the lipase enzyme with a percentage of 53.17% at 25 mg/mL and IC₅₀ of 19.44 ± 0.16 mg/mL. These findings suggest that the extract contains compounds capable of modulating the enzymatic activity involved in the digestion of carbohydrates and lipids, which may contribute to postprandial glycemic control. Therefore, the ethanolic extract of andiroba residue shows promise for antidiabetic strategies and highlights the sustainable use of industrial waste with pharmaceutical potential.

本研究探讨了皱籽楝（andiroba，Carapa guianensis）工业残渣乙醇提取物的生物活性潜力，重点关注其在酶抑制（enzyme inhibition）方面的药学应用价值。研究通过定量分析测定了样品中的总酚与总黄酮含量，并开展体外实验，采用DPPH法与FRAP法评估抗氧化活性，同时检测提取物对α-淀粉酶与脂肪酶的抑制作用。针对样品中总酚与总黄酮的定量分析未得到具有统计学意义的结果。不过，该提取物在DPPH法检测中展现出抗氧化活性：在10 mg/mL浓度下抑制率为45.16 ± 7.43%，半抑制浓度（IC₅₀）为11.57 ± 1.05 mg/mL。采用FRAP法检测时，该提取物同样具备抗氧化能力，其抗氧化抑制值为238.90 ± 8.78%，半抑制浓度为0.51 ± 0.07 mg/mL。此外，该提取物对α-淀粉酶表现出抑制活性：在25 mg/mL浓度下抑制率可达65.38%，半抑制浓度为17.52 ± 4.57 mg/mL。该提取物对脂肪酶亦展现出抑制作用，在25 mg/mL浓度下抑制率为53.17%，半抑制浓度为19.44 ± 0.16 mg/mL。上述研究结果表明，该提取物中含有可调节碳水化合物与脂质消化相关酶活性的化合物，或有助于餐后血糖控制。因此，皱籽楝工业残渣乙醇提取物在抗糖尿病策略中具备应用前景，同时也为具有药学潜力的工业废弃物的可持续利用提供了科学参考。