Replication data for: Serum metabolomic analysis of the dose-response effect of dietary choline in overweight male cats fed at maintenance energy requirements

The present study investigated how choline intake affects the metabolomic profile of overweight cats fed at maintenance energy. Overweight (n=14; body condition score:6-8/9) male adult cats were supplemented with five doses of choline in a 5x5 Latin Square design. Cats received a daily dose of choline on extruded food (3620 mg choline/kg diet) for three weeks at maintenance energy requirements (130 kcal/kgBW0.4). Doses were based on body weight (BW) and the daily recommended allowance (RA) for choline for adult cats (63 mg/kg BW0.67). Treatment groups included: Control (no additional choline, 1.2 x NRC RA, 77 mg/kg BW0.67), 2 x NRC RA (126 mg/kg BW0.67), 4 x NRC RA (252 mg/kg BW0.67), 6 x RA (378 mg/kg BW0.67), and 8 x NRC RA (504 mg/kg BW0.67). Serum was collected after an overnight fast at the end of each treatment period and analyzed for metabolomic parameters through nuclear magnetic resonance (NMR) spectroscopy and direct infusion mass spectrometry (DI-MS). Data were analyzed using GLIMMIX, with group and period as random effects, and dose as the fixed effect. Choline at 6 and 8 x NRC RA resulted in greater concentrations of amino acids and one-carbon metabolites (P < 0.05) betaine, dimethylglycine and methionine. Choline at 6 x NRC RA also resulted in greater phosphatidylcholine and sphingomyelin concentrations (P < 0.05). Supplemental dietary choline may be beneficial for maintaining hepatic health in overweight cats, as it may increase hepatic fat mobilization and methyl donor status.

本研究探究了维持能量饲喂条件下，胆碱摄入量对超重猫代谢组学特征的影响。选取14只体况评分(Body Condition Score, BCS)为6-8/9的成年公猫，采用5×5拉丁方设计设置5个梯度胆碱补充剂量。所有受试猫均按照维持能量需求（130 kcal/kg体重^0.4）饲喂膨化日粮，每个处理周期为三周，每日在日粮中添加胆碱，基础日粮胆碱含量为3620 mg/kg。胆碱补充剂量基于个体体重(Body Weight, BW)以及成年猫胆碱每日推荐摄入量(Recommended Allowance, RA)标准：63 mg/kg BW^0.67。具体处理组包括：对照组（无额外胆碱补充，相当于1.2×美国国家研究委员会(National Research Council, NRC)推荐摄入量，即77 mg/kg BW^0.67）、2×NRC RA（126 mg/kg BW^0.67）、4×NRC RA（252 mg/kg BW^0.67）、6×NRC RA（378 mg/kg BW^0.67）以及8×NRC RA（504 mg/kg BW^0.67）。每个处理周期结束后，对受试猫进行隔夜禁食并采集血清样本，通过核磁共振(Nuclear Magnetic Resonance, NMR)光谱法与直接注入质谱法(Direct Infusion Mass Spectrometry, DI-MS)分析代谢组学参数。数据分析采用GLIMMIX模型，以组别和周期作为随机效应，剂量作为固定效应。结果显示，6×和8×NRC RA剂量的胆碱可显著提升氨基酸及一碳代谢物（甜菜碱、二甲基甘氨酸与蛋氨酸）的浓度（P<0.05）；6×NRC RA剂量的胆碱还可显著提升磷脂酰胆碱与鞘磷脂的浓度（P<0.05）。膳食补充胆碱或可有益于超重猫的肝脏健康，因其可增强肝脏脂肪动员能力并提升甲基供体状态。