Metagenomic Data for Browning Peninsula and Mitchell Peninsula (sampled 2005)
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资源简介：
Sample collection:
All soils were collected in 2005 and stored at -80˚C prior to gDNA extraction using the FastDNA SPIN kit for soil (MP Biomedicals, NSW, Australia). Three soil samples were obtained from Mitchell Peninsula (66°31'S, 110°59'E) and three were obtained from Browning Peninsula (66°27'S, 110°32'E). Samples from both sites were selected along a transect, with specific information provided below.
Metagenome sequencing and assembly:
Metagenomic shotgun libraries were prepared from community gDNA extractions using the Nextera DNA Flex Library Prep Kit (Illumina Inc.). Sequencing was performed on an Illumina NextSeq500 platform with 2×150bp base pair high output run chemistry and 5GB coverage per sample.
Description of ACE Sample IDs (including in filenames) and the relevant soil samples:
* SB9748
- Soil barcode= 91062
- Original barcode= 91604
- Sample Site= Browning Peninsula
- Transect= 3
- Distance samples= 2m
- Concentration of DNA sent= 1.2ng/µL (required concentration)
- Volume DNA sent= 80µL
* SB9749
- Soil barcode= 91604
- Original barcode= 26587
- Sample Site= Browning Peninsula
- Transect= 3
- Distance samples= 102m
- Concentration of DNA sent= 1ng/µL (required concentration)
- Volume DNA sent= 80µL
* SB9750
- Soil barcode= 91606
- Original barcode= 26617
- Sample Site= Browning Peninsula
- Transect= 3
- Distance samples= 202m
- Concentration of DNA sent= 0.3ng/µL (required concentration)
- Volume DNA sent= 180µL
* SB9751
- Soil barcode= 91447
- Original barcode= 36260
- Sample Site= Mitchell Peninsula
- Transect= 2
- Distance samples= 2m
- Concentration of DNA sent= 0.86ng/µL (required concentration)
- Volume DNA sent= 80µL
* SB9752
- Soil barcode= 91477
- Original barcode= 36290
- Sample Site= Mitchell Peninsula
- Transect= 2
- Distance samples= 102m
- Concentration of DNA sent= 0.9ng/µL (required concentration)
- Volume DNA sent= 80µL
* SB9753
- Soil barcode= 91507
- Original barcode= 36320
- Sample Site= Mitchell Peninsula
- Transect= 2
- Distance samples= 202m
- Concentration of DNA sent= 1.9ng/µL (required concentration)
- Volume DNA sent= 80µL
提供机构：
AU_AADC
