Multiregional blood-brain barrier phenotyping identifies the prefrontal cortex as the most vulnerable region to ageing in mice - Dataset

This dataset consists of transmission electron microscopy (TEM) images of brain capillaries (diameter < 10 μm) from 23 C57Bl/6J mice. We analysed 12 young mice (6 female, 6 male) at 2-3-months of age and 11 aged mice (5 female, 6 male) at 18-20-months. Images from three brain regions are included: corpus callosum (CC), hippocampus (HC), and the prefrontal cortex (PFC). Brain regions of interest were sectioned to ultrathin 60 nm sections for electron microscopy. Imaging magnification was chosen to show each vessel and adjecent perivascular area to include blood-brain barrier-relevant subcomponents. TEM images were segmented and analysed using FIJI (ImageJ). We include the original TEM images and the segmentation files used for analyses. Reference DOI of the associated publication: 10.1093/braincomms/fcaf332本数据集涵盖23只C57Bl/6J小鼠的脑毛细血管（直径<10μm）透射电子显微镜（Transmission Electron Microscopy, TEM）图像。我们对12只年轻小鼠（雌性、雄性各6只）进行了分析，其月龄为2~3个月；同时纳入11只老年小鼠（雌性5只、雄性6只），月龄为18~20个月。 本数据集包含三个脑区的成像结果：胼胝体（corpus callosum, CC）、海马体（hippocampus, HC）以及前额叶皮层（prefrontal cortex, PFC）。所有感兴趣的脑区均被制备为60nm超薄切片以用于电子显微镜成像。成像倍率设置为可完整显示每一条血管及其邻近的血管周区域，从而涵盖与血脑屏障相关的所有亚结构组分。 本数据集的TEM图像通过FIJI（ImageJ）软件完成分割与分析，同时包含原始TEM图像及用于分析的分割文件。 相关研究论文的引用DOI为：10.1093/braincomms/fcaf332