Starvation increases susceptibility to bacterial infection and promotes systemic pathogen proliferation in Drosophila melanogaster females

Defense against pathogens and parasites requires substantial investment of energy and resources on part of the host. This makes the host immune function dependent on availability and accessibility of resources. A resource deprived host is therefore expected to be more susceptible to infections, although empirical results do not always align with this prediction. Limiting host access to resources can additionally impact within-host pathogen numbers, either directly by altering the amount of resources available to the pathogens for proliferation or indirectly by altering the efficiency of the host immune system. We tested for the effects of host starvation (complete deprivation of resources) on susceptibility to bacterial pathogens, and within-host pathogen proliferation, in Drosophila melanogaster females. Our results show that starvation increases post-infection mortality of the host, but in a pathogen-specific manner. This increase in mortality is always accompanied by increased within..., 1. Fly populations and general handling
Experiments reported here were carried out on flies from a large, outbred laboratory adapted population of Drosophila melanogaster, LH (Chippindale and Rice 2001, Prasad et al., 2007, Nandy et al., 2012). The LH population is maintained on a 14-day discrete generation cycle, at 25 OC temperature and 12:12 hour light-dark cycle, on cornmeal-molasses-yeast medium, at a census size of about 1900 adults. The flies are maintained in vials (95 mm height and 25 mm diameter); each generation starts with setting up of 60 vials with 150 eggs each on 8-10 ml of food medium. 12 days post-egg laying (PEL), by which time most adults have eclosed, adults from different vials are mixed together and redistributed into 60 vials with 16 females and 16 males in each vial. The vials are supplied with limiting live dietary yeast supplement, and on 14th day PEL, the adults are transferred to fresh vials and allowed to oviposit for 18 hours to start the next generation. ..., , # Starvation increases susceptibility to bacterial infection and promotes systemic pathogen proliferation in Drosophila melanogaster females

[https://doi.org/10.5061/dryad.pvmcvdnp8](https://doi.org/10.5061/dryad.pvmcvdnp8)

## Description of the data and file structure

The file (\"survival_data.xlsx\") was generated in 2018-2019 by Aabeer Basu and others at the Evolutionary Biology Lab, Department of Biological Sciences, IISER Mohali.

The file (\"bacterial_load_data.xlsx\") was generated in 2021 by Aabeer Basu and others at the Evolutionary Biology Lab, Department of Biological Sciences, IISER Mohali.

### Files and variables

#### File: README\_bacterialload.txt

**Description:** 1. Title of Dataset: \"Effect of resource limitation on host post-infection survival and systemic pathogenic growth\"

2\. Author Information

A. Principal Investigator Contact Information

Name: Prof. N. G. Prasad

Institution: Indian Institute of Science Education and Research, Mohali

Address: IISER Mohali, S...

宿主抵御病原体与寄生虫需要投入大量能量与资源，这使得宿主免疫功能依赖于资源的可获得性与可及性。因此，资源匮乏的宿主理应更易受到感染，但实证结果并不总能与这一预测相符。限制宿主获取资源，还可通过两种途径影响宿主体内的病原体数量：一是直接改变病原体增殖可利用的资源总量，二是间接改变宿主免疫系统的运作效率。我们以黑腹果蝇（Drosophila melanogaster）雌性成虫为研究对象，检验了宿主饥饿（完全剥夺资源）对细菌感染易感性以及宿主体内病原体增殖的影响。研究结果显示，饥饿会提升宿主感染后的死亡率，但这种效应具有病原体特异性。这种死亡率的升高始终伴随着宿主体内……

1. 果蝇种群与常规饲养
本研究中的实验所用果蝇来自一个大型、经实验室驯化的远交黑腹果蝇种群LH（Chippindale与Rice 2001，Prasad等人2007，Nandy等人2012）。该LH种群以14天为一个离散世代周期进行维持，饲养条件为：温度25℃，光暗周期12:12小时，培养基为玉米粉-糖蜜-酵母培养基，种群普查规模约为1900只成虫。果蝇饲养于试管中（高度95mm，直径25mm）；每一世代初始设置60个试管，每个试管接入150枚卵，培养基体积为8-10ml。产卵后12天（PEL），此时绝大多数成虫已经羽化，将不同试管中的成虫混合后重新分配至60个新试管中，每个试管放置16只雌性与16只雄性成虫。该试管会添加限量的活性膳食酵母补充剂；在产卵后第14天（PEL），将成虫转移至新鲜试管中，使其产卵18小时以启动下一世代。

# 饥饿提升黑腹果蝇雌性个体的细菌感染易感性并促进系统性病原体增殖
[https://doi.org/10.5061/dryad.pvmcvdnp8](https://doi.org/10.5061/dryad.pvmcvdnp8)

## 数据与文件结构说明
文件"survival_data.xlsx"由印度科学教育与研究学院莫哈利分校（IISER Mohali）生物科学系进化生物学实验室的Aabeer Basu等人于2018-2019年生成。
文件"bacterial_load_data.xlsx"由印度科学教育与研究学院莫哈利分校（IISER Mohali）生物科学系进化生物学实验室的Aabeer Basu等人于2021年生成。

### 文件与变量
#### 文件：README_bacterialload.txt
**描述：**1. 数据集标题："资源限制对宿主感染后存活情况与系统性病原增殖的影响"
2. 作者信息
A. 项目负责人联系方式
姓名：N. G. Prasad教授
所属机构：印度科学教育与研究学院莫哈利分校（IISER Mohali）
地址：IISER Mohali，S……