Dataset of 111 metagenome-assembled genomes from cattle manure, soil and manured soil samples
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Description of the data and file structure This data consists of fasta sequences and related information of 111 metagenome-assembled genomes from microcosms constructed with fresh manure of dairy cows under antibiotic prophylaxis and pasture soil of two organic farms. The data can also be accessed via NCBI BioProject PRJNA1231077 Files and variables File: MAGs_FASTA_files.zip Description: ZIP-compresed FASTA files of the MAGs File: MAGs_data.xlsx Description: Excel file containing MAGs information Variables name of MAGs origin (i.e., manure, soil or manured soil) and experiment (soil S or B) name of the original sample assembly type and binning method MAG filename taxonomic affiliations predicted using GTDB-Tk, including the best match taxonomy and % ANI to the closest placement if available quality metrics (completeness, contamination) detection of 5S, 16S and 23S rRNA genes tRNA amino acids coverage mapping reads % on average and the maximum in one sample assembly stats (GC content, N50, genome size, scaffold and contig count, N90, L50 and L90) NCBI information (i.e., SRA and BioSample accessions, coverage) 16S rRNA-based identification and sequence Access information Other publicly accessible locations of the data: NCBI BioProject PRJNA1231077 (https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1231077) Data was derived from the following sources: NCBI BioProject PRJNA743290 (https://www.ncbi.nlm.nih.gov/bioproject/PRJNA743290)
数据与文件结构说明 本数据集包含111个宏基因组组装基因组（metagenome-assembled genomes, MAGs）的FASTA序列及相关信息，这些基因组来源于以经抗生素预防处理的奶牛鲜粪构建的微宇宙体系，以及两家有机农场的牧场土壤样本。本数据集亦可通过NCBI BioProject（PRJNA1231077）获取。 文件与变量 文件：MAGs_FASTA_files.zip 描述：ZIP压缩格式的MAGs FASTA序列文件 文件：MAGs_data.xlsx 描述：包含MAGs相关信息的Excel表格文件 变量 - MAG名称 - 样本来源（即粪便、土壤或施肥土壤）与实验分组（土壤S组或B组） - 原始样本名称 - 组装类型与分箱方法 - MAG文件名 - 基于GTDB-Tk预测的分类学归属信息，包含最优匹配分类学结果，以及若可获取时与最邻近分类单元的平均核苷酸一致性（average nucleotide identity, ANI）百分比 - 质量评估指标（基因组完整性、污染率） - 5S、16S及23S核糖体RNA（ribosomal RNA, rRNA）基因检测结果 - tRNA对应的氨基酸种类 - 测序覆盖度 - 单样本平均比对reads百分比与单样本最大比对reads百分比 - 组装统计信息（GC含量、N50、基因组大小、scaffold与contig数量、N90、L50及L90） - NCBI相关信息（即序列读取存档（Sequence Read Archive, SRA）与生物样本（BioSample）编号、覆盖度） - 基于16S rRNA基因的物种鉴定结果及对应序列 获取途径 本数据集的其他公开获取渠道： NCBI BioProject PRJNA1231077（https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1231077） 本数据集衍生自以下数据源： NCBI BioProject PRJNA743290（https://www.ncbi.nlm.nih.gov/bioproject/PRJNA743290）
