Supplementary data: Role of miRNAs in the function of restoration of severe congenital neutropenia associated genes

Supplementary Figure S1. Visual representation of the regulatory network generated in BioNet UCR, by adding FORWARD and BACKWARD genes (HAX1, JAGN1, WAS, G6PC3) and deleting source and autoregulated nodes. Supplementary Figure S2. Differential expression analysis done in Cytoscape program to verify which nodes are regulated negatively or positively by their LogFC values. Supplementary Figure S3. Result of the parameter estimation task from CopasiUI applied to the model. Particle swarm algorithm was applied, showing a fitted model with no species going under the 0.05 threshold. Supplementary Table S4. HAX1 simulated mutation data related to its associated CNV MIR 6864, whereas 0.5 CNV is an approximate point where the function of the gene (concentration) can be restored. Supplementary Table S5. JAGN1 simulated mutation data related to its associated CNV MIR 6515, whereas 1.05 CNV is an approximate point where the function of the gene (concentration) can be restored. Supplementary Figure S6. Proposal of experimental design of an ex vivo cellular model, in which the results of the in silico model can be measured in a quantitative way.补充图S1：在BioNet UCR中构建的调控网络可视化结果，该网络通过添加正向与反向调控基因（HAX1、JAGN1、WAS、G6PC3），并删除源节点与自调控节点得到。 补充图S2：在Cytoscape软件中完成的差异表达分析，用于基于各节点的对数倍变化（LogFC）值验证其调控方向（正向或负向）。 补充图S3：将CopasiUI的参数估计任务应用于该模型所得的结果。本次分析采用粒子群算法，最终得到的拟合模型中所有物种的浓度均未低于0.05阈值。 补充表S4：针对其关联拷贝数变异（CNV）MIR 6864的HAX1模拟突变数据，其中当CNV为0.5时，该基因的功能（浓度水平）可得到恢复的近似临界点。 补充表S5：针对其关联CNV MIR 6515的JAGN1模拟突变数据，其中当CNV为1.05时，该基因的功能（浓度水平）可得到恢复的近似临界点。 补充图S6：体外（ex vivo）细胞模型的实验设计方案，该方案可定量检测计算机模拟（in silico）模型的预测结果。