Summary of captured CDRH3 diversity and analysis of libraries by NGS.

HnA displays naive human CDRH3, MnA naive mice CDRH3, MiB CDRH3 from mice immunized with hIFNγ, MiC CDRH3 from mice immunized with hCCL5, SnA is the initial synthetic library, SnA-R3 is the selection round used as a source of diversity to generate SiB. The section “size” informs about the number of transformants after electroporation for the libraries and the output of selection for SnA-R3. Sequencing data show the total number of VH sequences analyzed by NGS and the number of unique VH they contained, also expressed as a percentage of the total sequences.