n: number of samples; [Ct]: cycle threshold; Geo Mean [Ct]: geometric mean of Ct value; ar Mean [Ct]: arithmetic mean of Ct value; Min and Max [Ct]: extreme values of Ct; Min and Max [x-fold]: extreme
NAMPT (nicotinamide phosphoribosyltransferase, also known as PBEF, visfatin); p21; housekeeping genes beta-ACTIN, TBP (TATA-box-binding protein) and HPRT (hypoxanthine phophoribosyltransferase).
Reverse transcription quantitative real-time PCR (RT-qPCR) is a popular adopted technique to detect gene expression, and the selection of appropriate reference genes is crucial for data normalization.