Data from: The cell uptake properties and hyperthermia performance of Zn0.5 Fe 2.5O4 /SiO2 nanoparticles as magnetic hyperthermia agents

Zn0.5Fe2.5O4 nanoparticles (NPs) of 22nm are synthesized by one-pot
approach and coated with silica for magnetic hyperthermia agents. The
nanoparticles exhibit superaramagnetic characteristics, high specific
absorption rate (SAR) (1083 wg-1, f=430 kHz, H=27 kAm-1), large saturation
magnetization (Ms=85emug-1), excellent colloidal stability and low
cytotoxicity. The cell uptake properties have been investigated by
Prussian blue staining, transmission electron microscope (TEM) and
inductively coupled plasma mass spectrometer (ICP-MS), which resulted in
time dependent as well as concentration dependent internalization. The
internalization appeared between 0.5h to 2h, the NPs were mainly located
in the lysosomes and kept good dispersion after incubation with human
osteosarcoma MG-63 cells. Then, the relationship between cell uptake and
magnetic hyperthermia performance was studied. Our results showed that the
hyperthermia efficiency rest with the amount of internalized NPs, which
was depended on the concentration and incubation time. Interestingly, the
NPs could still induce tumor cells to apoptosis/necrosis when
extracellular NPs were rinsed, but the cell kill efficiency is lower than
no rinse groups, which indicate that local temperature rise rather than
mechanical mechanism is the main factor that induce tumor cells death. Our
findings suggest that this high SAR and biocompatible silica coating
Zn0.5Fe2.5O4 nanoparticles could serve as a new agents for magnetic
hyperthermia.