BIOGRID CURATED DATA FOR PUBLICATION: Control of mitotic spindle position by the Saccharomyces cerevisiae formin Bni1p.

Protein-Protein, Genetic, and Chemical Interactions for Lee L (1999):Control of mitotic spindle position by the Saccharomyces cerevisiae formin Bni1p. curated by BioGRID (https://thebiogrid.org); ABSTRACT: Alignment of the mitotic spindle with the axis of cell division is an essential process in Saccharomyces cerevisiae that is mediated by interactions between cytoplasmic microtubules and the cell cortex. We found that a cortical protein, the yeast formin Bni1p, was required for spindle orientation. Two striking abnormalities were observed in bni1Delta cells. First, the initial movement of the spindle pole body (SPB) toward the emerging bud was defective. This phenotype is similar to that previously observed in cells lacking the kinesin Kip3p and, in fact, BNI1 and KIP3 were found to be in the same genetic pathway. Second, abnormal pulling interactions between microtubules and the cortex appeared to cause preanaphase spindles in bni1Delta cells to transit back and forth between the mother and the bud. We therefore propose that Bni1p may localize or alter the function of cortical microtubule-binding sites in the bud. Additionally, we present evidence that other bipolar bud site determinants together with cortical actin are also required for spindle orientation.

蛋白质-蛋白质、遗传和化学相互作用数据集，源自 Lee L (1999) 的《通过酿酒酵母formin Bni1p调控有丝分裂纺锤体位置》：细胞分裂过程中，有丝分裂纺锤体与细胞分裂轴的对齐是一个至关重要的过程，该过程由细胞质微管与细胞质膜的相互作用介导。本研究发现，细胞质膜蛋白酵母formin Bni1p对于纺锤体定向至关重要。在bni1Δ细胞中观察到两种显著的异常现象。首先，纺锤体极体（SPB）向新形成的芽的初始移动存在缺陷，这一表型与先前在缺乏驱动蛋白Kip3p的细胞中观察到的表型相似，实际上，BNI1和KIP3被发现处于相同的遗传途径中。其次，微管与细胞质膜之间异常的拉动相互作用似乎导致bni1Δ细胞中的前有丝分裂纺锤体在母细胞和芽之间来回移动。因此，我们提出Bni1p可能定位或改变芽中细胞质膜微管结合位点的作用。此外，我们还提供了证据表明，其他双极芽位确定因子以及细胞质肌动蛋白也是纺锤体定向所必需的。