five

Real- time quantiative PCR analysis of HSC derived from sleep deprived mice

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NIAID Data Ecosystem2026-03-11 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE48144
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HSC were derived from mice sleep deprived for 4 hours and compared to mice that slept for 4 hours during the same circadian time. 5 mice were pooled for each sample and bones from femur, tibia and spine were crushed and stained to lineage deplete and enrich for ckit+, sca-1+,Flk-,CD150+, CD34- qpcr miRNA profiling from approximately 50ng total RNA extracted from HSC FACS sorted into Trizol.
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2019-10-29
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