Rearing environment and genotype convey kinship by shaping gut microbiota and odour profiles in Drosophila melanogaster.

To investigate the role of the larval environment (i.e. diet) and relatedness on the gut microbiota of Drosophila melanogaster, non-virgin females laid eggs, during 36 h and consecutively, in vials with three different mediums (banana, carrot and corn). This design allowed us to compare full siblings raised in different larval environments. At emergence, we collected virgin males (i.e. experimental flies), from which we used a pool of 5 flies for each sample of analysis. For all pools of samples (n=61), the 16S rRNA gene analysis of the bacterial community were carried out. PCR amplification of the V3-V4 regions were performed and sequencing of the amplicons using the Illumina MiSeq with 300 bp x2 chemistry.