qRT-PCR Primers and Supplementary Images for Melasma Study

This dataset contains supplementary materials for the study "Fractional Microneedle Radiofrequency Inhibits Melanin Metabolism, Microenvironmental Inflammation, and Senescent Fibroblasts in Melasma."

The dataset includes two files:

1. Excel file (Supplemental_online_material.xlsx): Contains forward and reverse primer sequences for 16 genes analyzed by quantitative real-time PCR (qRT-PCR).

Melanogenesis-related genes:
- Tyr (Tyrosinase)
- Mitf (Microphthalmia-associated transcription factor)
- Trp-1 (Tyrosinase-related protein 1)

Pro-melanogenic factors:
- Hgf (Hepatocyte growth factor)
- Scf (Stem cell factor)
- Gdf-15 (Growth differentiation factor 15)

Inflammation-related genes:
- Tlr-2 (Toll-like receptor 2)
- Tlr-4 (Toll-like receptor 4)
- Il-17 (Interleukin 17)
- Cox-2 (Cyclooxygenase 2)

Angiogenesis-related gene:
- Vegf (Vascular endothelial growth factor)

Photoaging-related genes:
- Mmp-1 (Matrix metallopeptidase 1)
- Mmp-2 (Matrix metallopeptidase 2)
- Mmp-3 (Matrix metallopeptidase 3)
- Mmp-9 (Matrix metallopeptidase 9)
- Collagen I

All primers were designed and synthesized by Shanghai Sangon Biotech Co., Ltd. for use with guinea pig (Cavia porcellus) tissue samples. These sequences correspond to the methods described in Section 2.6 (Quantitative Real-Time PCR) of the manuscript.

2. Word document (Supplemental_online_material.docx): Contains supplementary images for Figure 1, including 2 high-resolution photographs (1024×1280 pixels, JPEG format) showing the experimental setup and treatment areas on guinea pig dorsal skin.

File formats:
- Microsoft Excel (.xlsx) - Primer sequences
- Microsoft Word (.docx) - Supplementary images for Figure 1

Species: Guinea pig (Cavia porcellus)
Total number of primer pairs: 16
Total number of supplementary images: 2