Gene knockdown in tick midguts and salivary glands.
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Twenty female ticks were injected with gene-specific dsRNA or Rs86 control dsRNA. Ten ticks per group were collected after 7 days of feeding and midguts and salivary glands dissected for RNA extraction to characterize gene knockdown by real-time RT-PCR with respect to Rs86 control. The mRNA levels were normalized against tick 16S rRNA and cyclophilin and compared between test dsRNA-treated ticks and controls treated with Rs86 dsRNA by Student's t-test with unequal variance (*PGene knockdown in tick midguts and salivary glands.
创建时间：
2015-12-03
