Untargeted metabolomics peak intensity on CIA mouse plasma

Untargeted metabolomics with ultrahigh performance liquid chromatography-tandem mass spectroscopy (UPLC-MS/MS) was done on plasma of collagen-induced arthritis (CIA) mouse model. Data keys and explanations are below. mouseID: Mouse ID for the current study Treatment: CIA or control Time: Time of sacrifice and sample collection Intensity: Metabolite peak intensity measured by UPLC-MS/MS CHEM_ID: Internal ID for metabolite SUPER_PATHWAY: The general biochemical class assigned to the identified biochemical (e.g. lipid, amino acid, carbohydrate, etc.) intended for sorting compounds by broad biochemical classes SUB_PATHWAY: A more specific biochemical class assigned to the identified biochemical (e.g. TCA cycle, monoacylglycerols, etc.) intended for grouping/sorting compounds by biochemical pathway/class The rest of the data keys includes metabolite identifiers in multiple public databases and analysis platform.本研究针对胶原诱导性关节炎（collagen-induced arthritis, CIA）小鼠模型的血浆样本，采用超高效液相色谱-串联质谱（ultrahigh performance liquid chromatography-tandem mass spectroscopy, UPLC-MS/MS）开展非靶向代谢组学分析。以下为各数据字段及其详细说明： mouseID：小鼠编号，即本研究中受试小鼠的唯一标识 Treatment：处理分组，分为胶原诱导性关节炎模型组（CIA）与正常对照组 Time：取样时间，即处死小鼠并采集血浆样本的时间节点 Intensity：代谢物峰强度，指通过UPLC-MS/MS测得的代谢物色谱峰信号强度 CHEM_ID：代谢物内部编号，为本研究鉴定出的代谢物分配的内部唯一识别码 SUPER_PATHWAY：一级生化类别，为已鉴定代谢物划分的宽泛生化分类（如脂质、氨基酸、碳水化合物等），用于按大类对化合物进行分类与排序 SUB_PATHWAY：二级生化类别，为已鉴定代谢物划分的更细分生化分类（如三羧酸循环、单酰甘油类等），用于按生化通路/类别对化合物进行分组与排序 剩余数据字段涵盖多个公共数据库及分析平台中的代谢物标识符。