EU incorporation in Ccnc/Cdk8 WT/KO mESCs
收藏NIAID Data Ecosystem2026-03-12 收录
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http://flowrepository.org/id/FR-FCM-Z3EQ
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资源简介：
To assess the impact of Ccnc or Cdk8 loss on global transcription, measured by the incorporation of the nucleotide analogue 5-ethynyl uridine into RNA. Co-incubation with the RNAPI inhibitor (10uM 3h CX5641) was used to remove nucleolar signal and specifically assess RNAPII (and RNAPIII)-mediated transcription.
Conclusion:
No discernible impact of Ccnc or Cdk8 loss. No EdU controls for baseline levels. Co-incubation with 3h 5uM ActD as a positive control for total transcription inhibition
创建时间：
2021-01-01
