Table 1 in Application of the NucliSENS easyMAG system for nucleic acid extraction: optimization of DNA extraction for molecular diagnosis of parasitic and fungal diseases

Table 1. Quantification of DNA following extraction from stool samples (Cryptosporidium and Giardia) and white blood cell samples (Leishmania). This table presents the number of positive experiments and the mean number of molecular targets detected from four different samples for each parasite. The same quantity of each sample was tested with and without FastPrep disruption. Without disruptionAfter FastPrep disruptionPositive PCRMean quantity Cells/tubePositive PCRMean quantity Cells/tubep valueCryptosporidium parvum4/45474/419400.03Giardia intestinalis2/49.54/46360.005Leishmania infantum4/43094/4285NS表1 粪便样本（隐孢子虫属（Cryptosporidium）与贾第鞭毛虫属（Giardia））及白细胞样本（利什曼原虫属（Leishmania））提取后的DNA定量分析。本表格展示了每种寄生虫的4份独立样本的阳性实验结果数，以及检测到的分子靶标平均数量。每份样本采用等量样品，分别在未经破碎处理与经FastPrep破碎处理两种条件下开展检测。表格列依次为：寄生虫种类、未经破碎处理组的阳性聚合酶链式反应（Polymerase Chain Reaction，PCR）数、每管平均细胞数、经FastPrep破碎处理组的阳性PCR数、每管平均细胞数、p值。具体数据如下：1. 微小隐孢子虫（Cryptosporidium parvum）：未经破碎处理组阳性PCR数为4/4，每管平均细胞数为547；经FastPrep破碎处理组阳性PCR数为4/4，每管平均细胞数为19400，p值为0.03。2. 肠道贾第鞭毛虫（Giardia intestinalis）：未经破碎处理组阳性PCR数为2/4，每管平均细胞数为9.5；经FastPrep破碎处理组阳性PCR数为4/4，每管平均细胞数为6360，p值为0.005。3. 婴儿利什曼原虫（Leishmania infantum）：未经破碎处理组阳性PCR数为4/4，每管平均细胞数为309；经FastPrep破碎处理组阳性PCR数为4/4，每管平均细胞数为285，p值为无统计学意义（NS）。