The effect of pH and ionic strength on sfGFP spectroscopic characteristics.
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Absorption spectra (A) and fluorescence spectra (B) of sfGFP in the solutions with different pH. Fluorescence was excited at 390 nm and normalized to total fluorescence at current pH value. The numbers on panels A and B are the values of pH in solution. Absorption spectra (C) and fluorescence spectra (D) of sfGFP in the buffered solution and in solutions with the equal ionic strength but containing 0.7 M Na2SO4 or 2.1 M NaCl. Fluorescence was excited at 390 nm and normalized in the same manner as data on panel B.
创建时间：
2014-10-27
