scRNAseq of two medulloblastoma cell lines (ONS-76 and HDMB-03) grown in 3D hydrogel culture. scRNAseq of two medulloblastoma cell lines (ONS-76 and HDMB-03) grown in 3D hydrogel culture

Two medulloblastoma cell lines (ONS-76 and HDMB-03) were grown in 3D hyaluronan hydrogels for three weeks. We observed nodules forming showing different behavior and wanted to evaluate if these different nodules (slow vs fast vs non-growing, migrating and invading cells) are also characterised by different gene expression patterns. We performed this experiment on a SHH (ONS-76) and on a group 3 MB (HDMB-03) cell line to compare if certain subpopulations would be unique for the subgroups.