Code analysis and supplementary files for "Molecular responses to Marteilia sydneyi infection in the Sydney rock oyster Saccostrea glomerata"

This dataset contains the de novo transcriptome assembly, gene and protein prediction and annotation, R scripts and workflows used to analyse differential gene expression in Sydney rock oysters (Saccostrea glomerata) infected with the parasite Marteilia sydneyi. The code includes differential expression analysis using DESeq2, gene ontology (GO) enrichment analysis, and visualization of results. These analyses were conducted as part of a study investigating the molecular mechanisms underlying oyster immune responses to parasitic infections. The provided scripts are essential for reproducing the results presented in the associated research paper "Molecular responses to Marteilia sydneyi infection in the Sydney rock oyster Saccostrea glomerata" and serve as a resource for researchers conducting transcriptomic studies on marine invertebrates. For additional analysis such as de-novo transcriptome assembly, please see the linked repository URL below under "Repository URL." For sequencing data please see the following link: http://www.ncbi.nlm.nih.gov/bioproject/1173877本数据集涵盖针对感染寄生虫Marteilia sydneyi的悉尼岩牡蛎（Saccostrea glomerata）开展差异基因表达分析所需的从头转录组组装（de novo transcriptome assembly）、基因与蛋白质预测及注释流程，配套R脚本与分析工作流。该代码涵盖基于DESeq2的差异表达分析、基因本体（Gene Ontology, GO）富集分析，以及研究结果可视化相关内容。上述分析均为一项研究的组成部分，该研究旨在探究牡蛎应对寄生虫感染的免疫反应背后的分子机制。本数据集提供的脚本可复现相关研究论文"Molecular responses to Marteilia sydneyi infection in the Sydney rock oyster Saccostrea glomerata"中的实验结果，同时可为开展海洋无脊椎动物转录组学研究的科研人员提供参考资源。 如需开展从头转录组组装等额外分析，请参阅下方"Repository URL"项下的关联仓库链接。 如需获取测序数据，请访问以下链接：http://www.ncbi.nlm.nih.gov/bioproject/1173877