H-45 WGS vs IWGSC RefSeq v1.0 Genome Assembly
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https://researchdata.edu.au/h-45-wgs-genome-assembly/1604022
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资源简介:
WGS reads of H-45 (http://dx.doi.org/10.1111/j.1467-7652.2012.00717.x) were aligned against the IWGSC Triticum aestivum Chinese Spring RefSeq v1.0 genome assembly using Minimap2. These BAM files (.bam) and index files (.bam.bai) are provided together with a summary of read alignment coverage bigWig files (.bam.bw). SNP variants were called from these BAM files to generate VCF files (.bam.vcf.gz) and index files (.bam.vcf.gz.tbi) and are provided together with a summary of SNP density (SNPs per 10 kbp) bigWig files (.bam.vcf.w10000_s10000.bw). VCF files contain the following filter values and corresponding meaning: PASS = high quality (Q>=30) homozygous; Het = high quality (Q>=30) heterozygous; LowQualHom = low quality (Q
H-45的全基因组测序(Whole Genome Sequencing, WGS)读段(来源链接:http://dx.doi.org/10.1111/j.1467-7652.2012.00717.x)使用Minimap2比对至国际小麦基因组测序联盟(International Wheat Genome Sequencing Consortium, IWGSC)的普通小麦*Triticum aestivum*中国春参考基因组组装版本v1.0。本数据集提供了比对得到的BAM文件(Binary Alignment Map, .bam)及其索引文件(.bam.bai),同时附带了读段比对覆盖度汇总bigWig文件(.bam.bw)。基于上述BAM文件调用单核苷酸多态性(Single Nucleotide Polymorphism, SNP)变异位点,生成了VCF文件(Variant Call Format, .bam.vcf.gz)及其索引文件(.bam.vcf.gz.tbi),同时附带了SNP密度(每10千碱基对的SNP数目)汇总bigWig文件(.bam.vcf.w10000_s10000.bw)。VCF文件包含以下过滤值及其对应含义:PASS = 高质量(Q≥30)纯合变异;Het = 高质量(Q≥30)杂合变异;LowQualHom = 低质量(Q
提供机构:
The University of Adelaide



