Proteomic identification of metabolic changes in Paracoccidioides brasiliensis induced by a nitroheteroarylchalcone - Supplementary files

Aim: To access the metabolic changes caused by a chalcone derivative (LabMol-75) through a proteomic
approach. Methods: Proteomic analysis was performed after 9 h of Paracoccidioides brasiliensis yeast
(Pb18) cell incubation with the LabMol-75 at MIC. The proteomic findings were validated through in vitro
and in silico assays. Results: Exposure to the compound led to the downregulation of proteins associated
with glycolysis and gluconeogenesis, β-oxidation, the citrate cycle and the electron transport chain.
Conclusion: LabMol-75 caused an energetic imbalance in the fungus metabolism and deep oxidative stress.
Additionally, the in silico molecular docking approach pointed to this molecule as a putative competitive
inhibitor of DHPS.