PTPσ Novel Object Recognition

Novel Object Recognition test. Male and female mice 3-4 months old were used for these experiments. NOR test was performed in a transparent arena (39x20x16 cm) where two identical objects (white plastic ping-pong balls glued to 50-ml Falcon tube caps) holders at the bottom) were located (adapted from (Casarotto et al., 2021)). The animals were allowed to explore the objects for 15 min in 3 consecutive days (training sessions). At the test session (4 hours or 5 days after the last training session to assess short-term or long-term memory, respectively) one of the old objects was replaced by a new one (black rubber squash ball glued to 50-ml Falcon tube caps), and the animals were allowed to explore both objects for 5 min. The number of interactions with the objects was calculated, and the difference between the visits to the new object and the old object (fB-fA) was used to assess memory. Total number of visits (fA+fB) was used to assess total exploratory activity. Two different cohorts of animals were used to assess short-term and long-term memory.

新颖物体识别（Novel Object Recognition, NOR）实验。本实验采用3~4月龄的雌雄小鼠开展。实验在尺寸为39×20×16 cm的透明敞箱中进行，箱内放置两个完全相同的物体——固定于50 ml Falcon离心管盖底部的白色塑料乒乓球（该装置改编自Casarotto等，2021年的研究）。实验动物连续3天接受训练，每日可自由探索物体15分钟。测试阶段分别设置在最后一次训练后的4小时（用于评估短期记忆）或5天（用于评估长期记忆），此时将其中一个旧物体替换为固定于50 ml Falcon离心管盖底部的黑色橡胶壁球，随后允许小鼠探索两个物体共5分钟。研究人员统计小鼠与物体的互动次数，以新旧物体的探索次数差值（fB-fA）评估记忆能力，以总探索次数（fA+fB）衡量总探索活跃度。本研究使用两批独立的动物队列分别完成短期记忆与长期记忆的评估。