FACS-seq characterization of Cbe pfl ZTP riboswitch fitness landscape

Characterization of the tradeoff between sensitivity and dynamic range mediated by the sequence of the Cbe pfl ZTP riboswitch terminator loop. A library of riboswitch GFP reporter plasmids with randomized loop regions was characterized by FACS-seq in the presence of different concentrations of AICAR, allowing the construction of dose-response curves for hundreds of loop variants.