<i>Leptospira</i> strains used in the present study and results of the real time PCR assays using the species-specific probes and flanking primers.

1INIAV - Instituto Nacional de Investigação Agrária e Veterinária, Lisbon, Portugal. IHMT - Instituto de Higiene e Medicina Tropical, Lisbon, Portugal. KIT - Royal Tropical Institute, Amsterdam, The Netherlands; 2Set 1 targets the lipL32 gene of pathogenic Leptospira spp.; 3Set 2 targets the secY gene of L. interrogans; 4Set 3 targets the ompL1 gene of L. borgpetersenii; 5Set 4 targets the secY gene of L. kirschneri; 6Set 5 targets the secY gene of L. noguchii; nd - not done; Amplification (+) or no amplification (−). Leptospira strains used in the present study and results of the real time PCR assays using the species-specific probes and flanking primers.