Endogenous germline nuclear RNAi-mediated transcriptional silencing and histone H3 lysine 9 methylation in defined regions of the Caenorhabditis elegans genome. Caenorhabditis elegans

We describe the identification and characterization of genomic regions that are targeted by germline nuclear RNAi in C. elegans for transcriptional silencing and heterochromatic response. Overall design: We compared the wild type and germline nuclear Argonaute protein hrde-1 mutant for their RNA polymerase II ChIP-seq, H3K9 trimethylation ChIP-seq, pre-mRNA seq, mRNA-seq, and small RNA-seq profiles.