Pulse chase 4SU labeling - DCP1a and DCP1b KO cells

NIAID Data Ecosystem2026-05-02 收录

RNA代谢动力学

脉冲追踪标记

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https://www.ncbi.nlm.nih.gov/sra/SRP491868 数据链接链接失效反馈

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资源简介：

DCP1a and DCP1b were knocked out in HCT116 cell. Pulse-chase labeling experiment with 4SU was conducted in these cells and control cells.

创建时间：

2024-09-04

Thiol-linked alkylation for the metabolic sequencing of RNA [Transcriptional inhibition by Actinomycin D]. Mus musculus

RNA代谢动力学

表观转录组学

Gene expression profiling by high-throughput sequencing reveals qualitative and quantitative changes in RNA species at steady-state but obscures the intracellular dynamics of RNA transcription, proces

NIAID Data Ecosystem120

Pulse chase 4SU labeling - DCP1a and DCP1b KO cells

RNA代谢动力学

基因敲除功能验证

DCP1a and DCP1b were knocked out in HCT116 cell. Pulse-chase labeling experiment with 4SU was conducted in these cells and control cells.

NIAID Data Ecosystem50

Data to reproduce Figures 1-3 and associated supplemental figures

RNA代谢动力学

无义介导mRNA降解

Cellular RNA levels are a product of synthesis and degradation kinetics, which can differ among transcripts of the same gene. An important cause of isoform-specific decay is the nonsense-mediated mRNA

Figshare2025-03-25 更新10

Thiol-linked alkylation for the metabolic sequencing of RNA [SLAM-seq pulse/chase labeling in wildtype mES cells]. Mus musculus

RNA代谢动力学

表观转录组学

NIAID Data Ecosystem60

Solid phase chemistry to covalently and reversibly capture thiolated RNA

固相RNA捕获

RNA代谢动力学

Here, we describe an approach to enrich newly transcribed RNAs from primary mouse neurons using 4-thiouridine (s4U) metabolic labeling and solid phase chemistry. This one-step enrichment procedure cap

NIAID Data Ecosystem10

Pulse chase 4SU labeling - DCP1a and DCP1b KO cells

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