Data from: Balanced replacement of fish meal with Hermetia illucens meal allows efficient hepatic nutrient metabolism and increased fillet lipid quality in gilthead sea bream (Sparus aurata) juveniles

In the present study, gilthead sea bream (<em>Sparus aurata</em>) juveniles were reared using sustainable feeds containing insect meal from <em>Hermetia illucens</em> larvae and poultry by-products meal. Proteomics and Proton Nuclear Magnetic Resonance-based metabolomics analysis were used to assess the metabolic impact of tested dietary formulations in sea bream liver, whereas the composition of muscle fillet was characterized by means of metabolomics and gas chromatography of fatty acids methyl esters. Replacing fish meal with insect meal in a 5% fish meal diet did not substantially alter metabolism of dietary nutrients, leading to small but statistically detectable effects solely on lauric acid content of sea bream fillet, and few alterations in some markers of immune response, such as leukocyte elastase inhibitor-like, granzyme B (G,H)-like, and two associated ortholog groups namely serpin B, and chymase). Liver morphology confirmed the absence of structural damage or inflammation in the insect meal-fed group, which showed a lower amount of hepatic lipid deposition and accumulation, too.本研究以金头鲷（<em>Sparus aurata</em>）幼鱼为实验对象，采用含有黑水虻（<em>Hermetia illucens</em>）幼虫粉与家禽副产品粉的可持续饲料进行饲养。本研究采用蛋白质组学与基于质子核磁共振（Proton Nuclear Magnetic Resonance）的代谢组学分析手段，评估受试饲料配方对金头鲷肝脏的代谢影响；同时通过代谢组学与脂肪酸甲酯气相色谱法，对金头鲷肌肉鱼片的组成进行表征。在5%鱼粉饲料中以昆虫粉替代鱼粉，并未显著改变膳食营养素的代谢过程，仅对金头鲷鱼片的月桂酸含量产生了微小但具有统计学显著性的影响，且仅在部分免疫应答标志物上存在少量改变，例如类白细胞弹性蛋白酶抑制剂、类颗粒酶B（G,H），以及两个相关同源基因簇：丝氨酸蛋白酶抑制剂B（serpin B）与糜酶（chymase）。肝脏组织形态学分析证实，饲喂昆虫粉的实验组未出现组织结构损伤或炎症反应，同时该组的肝脏脂质沉积与蓄积水平也更低。