Enhancing PTH Measurement in Chronic Kidney Disease Patients: Unveiling a New Era with Liquid Chromatography Coupled with Mass Spectrometry in Tandem (LC-MS/MS)

These are the raw data (Excel and Medcalc files) of the abovementionned article. In brief, we have calibrated five immunoassays for PTH with a candidate LC-MS/MS reference method on a panel of 39 pooled samples. Then we have verified the robustness of this calibration with two different sets of samples from non-CKD, CKD and HD patients measured at two timepoints and compared the recalibrated immunoassays vs. the results we obtained with the LC-MS/MS. Our results show that recalibrated immunoassays align correctly together and with the LC-MS/MS.

本数据集为上述研究文章的原始数据，包含Excel文件与Medcalc文件。简言之，本研究采用候选液相色谱-串联质谱（Liquid Chromatography-Tandem Mass Spectrometry, LC-MS/MS）参考方法，针对39份混合样本组，对5种甲状旁腺激素（Parathyroid Hormone, PTH）免疫测定方法完成了校准。随后，我们使用来自非慢性肾脏病（Chronic Kidney Disease, CKD）、慢性肾脏病患者及血液透析（Hemodialysis, HD）患者的两套不同样本，在两个时间点进行检测，验证了该校准方案的稳健性，并将重新校准后的免疫测定结果与LC-MS/MS检测结果进行了对比。研究结果表明，重新校准后的免疫测定方法彼此之间以及与LC-MS/MS检测结果均保持一致。