Placental whole genome RNA sequencing dataset of monochorionic diamniotic twin pregnancies with and without selective fetal growth restriction

To compare placental transcriptomes between selective fetal growth restriction (sFGR) twins and normally grown twins (Control) in monochorionic diamniotic (MCDA) twin pregnancies, placentae from five sFGR and three control twin pairs were collected. Their placental transcriptomes were profiled by high throughput total whole genome RNA sequencing technology, which provides a global and deep examination of gene expression. The log2 ratio of the placental transcriptome expression levels of the smaller twin to that of the larger co-twin were calculated in each twin pair. Results in sFGR pregnancies were compared to that of the control pregnancies. Differentially expressed transcripts identified as the within-pair log expression ratio difference between sFGR and control groups were determined by Mann-Whitney U test. Based on these differentially expressed transcripts, biological pathways and upstream regulators were predicted using the Ingenuity Pathway Analysis (IPA) software. This dataset describes total 1429 differentially expressed placental transcripts, total 126 predicted biological pathways and 419 upstream regulators in sFGR. The data provide further insight in transcriptomic level to the pathomechansim of pregnancies complicated by sFGR. The dataset supports the research article entitled “Monochorionic twins with selective fetal growth restriction: insight from placental whole transcriptome analysis”.

为探究单绒毛膜双羊膜（monochorionic diamniotic, MCDA）双胎妊娠中，选择性胎儿生长受限（selective fetal growth restriction, sFGR）双胎与正常生长双胎（对照组）的胎盘转录组差异，本研究收集了5例sFGR双胎妊娠及3例对照双胎妊娠的胎盘组织。采用高通量全基因组RNA测序技术对上述胎盘组织的转录组进行测序分析，该技术可实现基因表达的全局性深度检测。针对每一双胎妊娠，计算较小胎儿与其同期较大同胞胎儿的胎盘转录组表达水平的log₂比值。将sFGR妊娠组的分析结果与对照组进行对比，通过曼-惠特尼U检验（Mann-Whitney U test）筛选出两组间双胎内基因表达对数比值存在显著差异的转录本（即差异表达转录本）。基于上述差异表达转录本，使用英格纽斯通路分析（Ingenuity Pathway Analysis, IPA）软件预测相关生物学通路及上游调控因子。本数据集共涵盖sFGR相关的1429个胎盘差异表达转录本、126条预测生物学通路及419个上游调控因子。本数据集从转录组层面为阐明合并sFGR的双胎妊娠的发病机制提供了新的研究视角，可为题为《单绒毛膜双胎合并选择性胎儿生长受限：胎盘全转录组分析的启示》的研究论文提供数据支撑。