Parabasalian species used for the gene identification and phylogenetic analyses.

aAbbreviations of the classes are: C, Cristamonadea; Tt, Tritrichomonadea; S, Spirotrichonymphea; H, Hypotrichomonadea; Tm, Trichomonadea; and Tn, Trichonymphea.bWhen the family name has changed in the new parabasalian classification [26], the corresponding former name [1] is also indicated in parenthesis. Abbreviations of the families are the following: L, Lophomonadidae; D, Devescovinidae; C, Calonymphidae; De, Deltotrichonymphidae; Sp, Spirotrichonymphidae; Hl, Holomastigotoididae; St, Staurojoeninidae; Tn, Trichonymphidae; Hp, Hoplonymphidae; E, Eucomonymphidae; Te, Teranymphidae; Di, Dientamoebidae; Tt, Tritrichomonadidae; M, Monocercomonadidae; and Tm, Trichomonadidae.cWe failed to identify the EF-1α gene in G. herculea.dThe GAPDH genes of Deltotrichonympha sp. and D. fragilis were unavailable.eThe EST data of P. hominis and the genome sequence of T. vaginalis[73] in the database were also used for phylogenetic analyses (see Materials and Methods).