Fig_5a_43Ind_SwitchBack_Strategy_iii

The microscopy images of the data in Fig. 5a for the following condition: 2 hours of CRISPRi induction (43% induction level) followed by inducers removal and the addition of 60 nM of AHL in the new medium. There are two data sets with three channels each: phase-contrast bright field, mVenus, mRFP. Data set 1 has 6 microscopy stage positions and data set 2 has 40 microscopy stage positions. The images were binned (2x2).

本数据集对应图5a中下述实验条件下的显微图像：经2小时CRISPRi诱导（诱导水平达43%）后，移除诱导剂并在新鲜培养基中加入60 nM的AHL。本数据集包含2个子集，每个子集均设有3个成像通道：相差明场（phase-contrast bright field）、mVenus、mRFP。其中子集1包含6个显微载物台成像位点，子集2包含40个显微载物台成像位点。所有图像均完成了2×2像素分箱处理。