Single-cell sequencing of peripheral blood mononuclear cells in acute Kawasaki disease

Kawasaki disease (KD) is the most common cause of acquired heart disease in children in developed countries. Based on single-cell RNA sequencing (scRNA-seq), we profiled peripheral blood mononuclear cells (PBMCs) from patients with acute KD before and after intravenous immunoglobulin (IVIG) therapy and from healthy controls. We identified the dynamics of cell abundance, gene expression patterns and biological processes underlying the immune dysregulation of each cell compartment. The single-cell landscape of both innate and adaptive immune responses provides new insights into pathogenesis and therapy of KD. PBMCs from KDs before and after IVIG therapy, and healthy controls were subjected to scRNA-seq, scBCR-seq and scTCR-seq with 10x Genomics. ------------------------------------------ Authors state the following: Raw data are deposited in the National Omics Data Encyclopedia (NODE) database (http://www.biosino.org/node/project/detail/OEP001162). Restrictions apply to the availability of these data, which are used under license for the current study and are not publicly available. Data are however available from the authors upon reasonable request and with permission of the NODE committee.