Galaxy-compatible AnnData object for pseudobulk analysis

This dataset is an AnnData object containing a curated subset of single-cell RNA-seq data. It includes non-classical monocytes (NCMs) and plasmacytoid dendritic cells (pDCs) derived from both bone marrow and peripheral blood samples. The dataset has been preprocessed and annotated with the metadata required for pseudobulk analysis, including cell type labels, condition, batch information, and tissue of origin. This subset has been prepared for educational purposes and is used to demonstrate pseudobulk aggregation and differential expression analysis in the Galaxy platform. A portion of the bone marrow data originates from the following publication: Rettkowski J, Romero-Mulero MC, Singh I, Wadle C, Wrobel J, Chiang D, Hoppe N, Mess J, Schönberger K, Lalioti ME, Jäcklein K, SilvaRego B, Bühler T, Karabacz N, Egg M, Demollin H, Obier N, Zhang YW, Jülicher C, Hetkamp A, Czerny M, Jones MJ, Seung H, Jain R, von Zur Mühlen C, Maier A, Lother A, Hilgendorf I, van Galen P, Kreso A, Westermann D, Rodriguez-Fraticelli AE, Heidt T, Cabezas-Wallscheid N."Modulation of bone marrow haematopoietic stem cell activity as a therapeutic strategy after myocardial infarction: a preclinical study."Nature Cell Biology. 2025 Apr;27(4):591–604.doi: 10.1038/s41556-025-01639-4PMID: 40175666 ; PMCID: PMC11991920本数据集为AnnData对象，包含经整理标注的单细胞RNA测序（single-cell RNA-seq）子集数据，包含源自骨髓与外周血样本的非经典单核细胞（non-classical monocytes, NCMs）与浆细胞样树突状细胞（plasmacytoid dendritic cells, pDCs）。 本数据集已完成预处理，并注释了伪批量分析（pseudobulk analysis）所需的元数据，包括细胞类型标签、实验条件、批次信息及组织来源。 该子集专为教学用途制备，用于演示Galaxy平台（Galaxy platform）上的伪批量聚合与差异表达分析流程。 该数据集的部分骨髓数据源自以下文献： Rettkowski J, Romero-Mulero MC, Singh I, Wadle C, Wrobel J, Chiang D, Hoppe N, Mess J, Schönberger K, Lalioti ME, Jäcklein K, SilvaRego B, Bühler T, Karabacz N, Egg M, Demollin H, Obier N, Zhang YW, Jülicher C, Hetkamp A, Czerny M, Jones MJ, Seung H, Jain R, von Zur Mühlen C, Maier A, Lother A, Hilgendorf I, van Galen P, Kreso A, Westermann D, Rodriguez-Fraticelli AE, Heidt T, Cabezas-Wallscheid N. 《心肌梗死发生后调控骨髓造血干细胞活性作为治疗策略的临床前研究》，《自然·细胞生物学》，2025年4月；27(4): 591–604. DOI: 10.1038/s41556-025-01639-4，PMID: 40175666，PMCID: PMC11991920