CRISPR-based epigenome editing screens identify transcriptional and epigenetic regulators of human CD8 T cell function [RNA-seq]

Human CD8+ T cells from three donors were transduced with lentivirus encoding for BATF3-2A-GFP or GFP and expanded for 10 days. Total RNA was isolated from each sample and submitted for RNA-seq. We then performed gene expression profiling analysis. Overall design: Comparative gene expression profiling analysis of RNA-seq data for human CD8 T cells (n = 3 donors) with or without BATF3 overexpression.