Plasmodium falciparum: Sequencing of mutagenized, drug-selected strains 019313R1 and 019313R2. Plasmodium falciparum

Plasmodium falciparum W2 parasites were mutagenized with ethyl methanesulfonate (EMS) and separated into a control population and drug-selected population. MMV019313 drug selection was applied to the drug-selected population at 600nM (approximately EC75) and maintained for 32 days, with no observable parasite growth. After 32 days of selection, drug pressure was removed. Parasites were observable after 7 days. WGS of drug-selected lines shows T-to-A mutation in the gene PF3D7_1128400 characterized as a bifunctional FPPS/GGPPS and resulting in a Ser228-to-Thr change in the protein.