The effect of COX2 inhibition on RAS-induced senescence

ER:RAS-G12V expressing IMR90 cells were treated with either 100nM 4-OHT or 40uM Celecoxib or both for 6 days leading to RAS-induced senescence (RIS) with or without COX2 inhibition. Overall design: IMR90 cells expressing a 4-hydroxytamoxifen (4-OHT) inducible estrogen receptor (ER)-coupled RAS-G12V (ER:RAS-G12V) were treated with either 100nM 4-OHT or 40uM Celecoxib or both for 6 days leading to RAS-induced senescence (RIS) with or without COX2 inhibition. Oligo-dT captured RNA was then used for library preparation and then analysed using mRNA-sequencing. There were 8 (eight) biological replicatesfor each of the experimental conditions. Untreated ER:RAS IMR90 cells were the control condition