Gene expression profile at single cell level of articular cartilage superficial cells from sham and dmm surgery mice and long bone periosteum cells from steady state mice.

Characteristics of the chondroprogenitors cells remain unidentified. Here we identify a chondroprogenitor population, which specifically expresses Procr,and used single cell RNA sequencing (scRNA-seq) and bulk RNA-seq to analyze the diversity of Prcor+ cells in articular cartilage and periosteum. Overall design: Articaular cartilage cells from sham and DMM surgery mice were isolated by Fluorescence-activatedcell sorting (FACS) with APC-conjugated CD31, CD45, and Ter119 antibody and analyzed using scRNAseq. Long bone periosteum cells from Prrx1-Cre;tdTomato;Col2.3-GFP were isolated by Fluorescence-activatedcell sorting (FACS) with APC-conjugated CD31, CD45, and Ter119 antibody and analyzed using scRNAseq. Articaular cartilage cells from steady state mice were isolated for Procr+CD105- and Procr+CD105+ cells, and analyzed using RNAseq.