Endogenous Ang1 localization in non-ischemic rat brains visualized with confocal laser microscopy.

(A) From left to right: markers of cells that make up the blood brain barrier (a, e, i, m; green), Ang1 (b, f, j, n; red), 4′,6-diamidino-2-phenylindole (DAPI) stain (c, g, k, o; blue), and a merged image (d, h, l, p). RECA1 is an endothelial cell marker protein; PDGFRβ is a pericyte marker; GFAP is an astrocyte marker; and MAP2 is a neuronal cell marker. RECA1, rat endothelial cell antigen; PDGFRβ, platelet-derived growth factor receptor β; GFAP, glial fibrillary acidic protein; MAP2, microtubule-associated protein 2; Ang1, angiopoietin-1. The scale bars are 10 µm. (B) Three-dimensional (3D) images of endogenous Ang1 in non-ischemic brains visualized with a confocal laser microscope. a: endothelial cell marker RECA1 (green) and endogenous Ang1 (red); b: neuronal cell marker MAP2 (green) and endogenous Ang1 (red). RECA1, rat endothelial cell antigen; Ang1, angiopoietin-1; MAP2, microtubule-associated protein 2. An arrow indicates Ang1 expressed in a pericyte. Arrowheads indicate Ang1 expressed in neuronal cells.