Analysis of IFN-I responses of human CD1c+ DC and monocytes by CITE-sequencing

Using CITE-sequencing, cell-type specific responses to type-I interferon stimulation were analysed across human CD1c+ dendritic cell and monocyte subsets, showing that IFN-ß drives the maturation of DC3 and the acquisition of a unique costimulatory profile characterized by high GITRL expression. Overall design: PBMC from three distinct human donors were cultured for 18h with or without recombinant human IFN-ß. Cells were then enriched for CD1c+ dendritic cells and monocytes, labeled with TotalSeq-A antibodies and partitioned using the 10X platform. The list of Total-seq A antibodies and their oligo tag sequence is available as a supplementary file.