CIL:1444

Rabbit psoas skeletal muscle fibers were held at constant length and rigor induced by immersion in glycerol. The fibers were quick-frozen by contact with a liquid helium- cooled copper block in a Heuser-type cryopress, and freeze-etched in a Balzers 400 freeze fracture machine. The sample was then rotary-replicated with Pt-C at 24 degrees and visualized in a JEOL 100CX2 transmission electron microscope operated at 100 kev. Images were recorded on Kodak 4489 film at 68,000x magnification. Stereo pairs were taken at +5 and -5 degrees. Films were scanned with a 20 micrometer pixel spacing on a Nikon Coolscan 9000ED scanner. stereo pair with CIL:1443

本数据集以兔腰大肌骨骼肌纤维为实验标本：将纤维固定于恒定长度状态，通过浸入甘油诱导肌僵直。随后将纤维与液氦冷却的铜块接触，借助Heuser型冷冻按压仪（Heuser-type cryopress）完成快速冷冻。接着将样品置于Balzers 400型冷冻断裂仪（Balzers 400 freeze fracture machine）中进行冷冻蚀刻，再以24°角采用铂-碳（Pt-C）对样品进行旋转复型。随后在加速电压为100 keV的JEOL 100CX2型透射电子显微镜（JEOL 100CX2 transmission electron microscope）下观察样品，以68000倍的放大倍率将图像记录于Kodak 4489胶片（Kodak 4489 film）上。以±5°倾角拍摄立体图像对，使用Nikon Coolscan 9000ED扫描仪（Nikon Coolscan 9000ED scanner）以20微米的像素间距对胶片进行扫描，该立体图像对编号为CIL:1443。