Characterising the proliferative drive of chronic lymphocytic leukemia (CLL) using mass cytometry (CyTOF)

We used CyTOF to study CLL proliferation and its possible connections to MYC and mTOR pathway activities at single cell resolution. We measured the abundance of 33 proteins and phosphorylated proteins, including markers for cell type, cell proliferation and signaling pathway activity 16 in primary CLLs. We exposed the tumors to CpG ODN (5ug/mL), with the mTOR inhibitor everolimus (250 nM), with CpG ODN and everolimus combined, and DMSO control to elicit proliferation and assess its dependence on mTOR. The raw CyTOF data (.fcs files) are provided in a compressed folder "fcs_files.zip" and sample metadata are provided in "metaData.tsv" file.